Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKSELIDAIAEKGGVSKTDAGKALDATIASITEALKKGDTVTLVGFGTFSVKERAARTGRNPKTGEELQIKATKVPSFKAGKGLKDSVA 4YF0 Chain:B ((2-91)) MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKKGDALQLVGFGTFKVNHRAERTG--PQTGKEIKIAAANVPAFVSGKALKDAVK

General information: TITO was launched using: RESULT: Template: 4YF0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 192 -9951 -51.83 -113.07 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.94 3D Compatibility (PKB) : -51.83 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.572

(partial model without unconserved sides chains): PDB file : Tito_4YF0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YF0-query.scw PDB file : Tito_Scwrl_4YF0.pdb: