Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKIYGIKNCNSMKKAFDVLQAKGLSYEFHDYKKQGIDADTLKIWLKEIGQDTVLNKKGTTWKKLSEEEQTRALSSEDHLIEALIAQPSLIKRPVLQTSKGFLVGFDETAYQNLSA 1RW1 Chain:A ((1-114)) TYVLYGIKACDTMKKARTWLDEHKVAYDFHDYKAVGIDREHLRRWCAEHGWQTVLNRAGTTFRKLDEAQKADL--DEAKAIELMLAQPSMIKRPVLELGGRTLVGFKPDAYAAALA

General information: TITO was launched using: RESULT: Template: 1RW1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 462 -62938 -136.23 -552.09 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -136.23 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.731

(partial model without unconserved sides chains): PDB file : Tito_1RW1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RW1-query.scw PDB file : Tito_Scwrl_1RW1.pdb: