Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKRTFLFTIIAATLSTVYAAPLTKDNGAPVGDNQNSTTAGANGATLLQDVQLIQKLQRFGRERIPERVVHARGTGVYGEFVSTKDLSDLTLASLFKAGTKTPVFVRFSTVIHPKGSPETARDPHGFAVKFYTQQGNWDLVGNNLPVFFIRDAIKFPDFVHAMKPDPVTNVQDPNRIFDFLQSQPWSINMLTYVYSNLGTAESYRTLDGFGVHAFKLYNDKGEYKYVKFNWRSQQGVKGLNLEQVREVQGRDWSHLTNDMYKNIYAGNYPKWDLYIQVLDPKDLDKFDFNPLDATKIWPNELVPEFKVGTLTLNRMPKNFFQETEQSAFAPGNLIPGIEPSEDRLLQGRVFSYSDTQLYRLGVNYQQIPVNRPRVAVNNNNQEGFMNMGQTESHVNYEPSQIEPKPATEKARAVQTPLEGTVMQHAIQKQQPYKQAGDLYRSYSALEKKDLIRNLAADLGQVKNVETKTVMLSYFYKADADYGTRLAKAAQLKDE 1M7S Chain:D ((2-472)) --------------------DTLTRDNGAVVGDNQNSQTAGAQGPVLLQDVQLLQKLQRFDRERIPERVVHARGTGVKGEFTASADISDLSKATVFKSGEKTPVFVRFSSVVHGNHSPETLRDPHGFATKFYTADGNWDLVGNNFPTFFIRDAIKFPDMVHAFKPDPRTNLDNDSRRFDFFSHVPEATRTLTLLYSNEGTPAGYRFMDGNGVHAYKLVNAKGEVHYVKFHWKSLQGIKNLDPKEVAQVQSKDYSHLTNDLVGAIKKGDFPKWDLYVQVLKPEELAKFDFDPLDATKIWPD--VPEKKIGQMVLNKNVDNFFQETEQVAMAPANLVPGIEPSEDRLLQGRVFSYADTQMYRLGANGLSLPVNQPKVAVNNGNQDGALNTGHTTSGVNYEPSRLEPRPADDKARYSELPLSGTTQQAKITREQNFKQAGDLYRSYSAKEKTDLVQKFGESLADTL-TESKNIMLSYLYKEDPNYGTRVAEVAKGDL-

General information: TITO was launched using: RESULT: Template: 1M7S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2325 -55891 -24.04 -118.66 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain D : 0.87 3D Compatibility (PKB) : -24.04 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.472

(partial model without unconserved sides chains): PDB file : Tito_1M7S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1M7S-query.scw PDB file : Tito_Scwrl_1M7S.pdb: