Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKIIHIDMDAFYASVELRERPDLRHLPVVISSHHPRAVIAAASYPAREFGLRSAMSMSQARKLCPQVVIIE-PNFEKYRAISAQIHSIFQQYTTLIEPLSLDEAYLDVTENLKQI----------------------------------ASATEVAMHIREDIFRQTGLTASAGVAPNKFLAKVASDWNKPNGLFVIKPSQVASFIQDL-PLKKIPGVGKVTQEKLQQLELHTLGDLQKIEEAVLVHHFGK-YGQQLYLYAQGIDNRPVQAERARQQISKETTFDSDFTLAQCQSYWHGLAEKVWQSLEKKQLNARGVNIKLKLKN-F---QTLQHSKSFKNP----IHSQ---QDLIQVLFLLLNEMHIPE--NFQFRLIGVLGFINYKLKLTTFSCLYGECCFFIQKNKF 3OSN Chain:A ((27-414)) SRVIVHVDLDCFYAQVEMISNPELKDKPLGVQQ---KYLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGEDLTRYREMSYKVTELLEEFSPVVERLGFDENFVDLTEMVEKRLQQLQSDELSAVTVSGHVYNNQSINLLDVLHIRLLVGSQIAAEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIKEIPGIGYKTAKCLEALGINSVRDLQTFSPKILEKELGISVAQRIQKLSFGEDNSPVILSGPPQSFSEEDSFKKCSSEVEAKNKIEELLASLLNRVCQDGRKPHTVRLIIRRYSSEKHYGRESRQCPIPSHVIQK---YDVMTPMVDILMKLFRN-MVNVKMPFHLTLLSVCFCNLK-----------------------

General information: TITO was launched using: RESULT: Template: 3OSN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1508 -13505 -8.96 -40.56 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -8.96 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_3OSN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OSN-query.scw PDB file : Tito_Scwrl_3OSN.pdb: