Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSVLTMAVNDSPITSVKTTQPLIALYCGSRAGNNPIYREKAIELASGIAEQGFGLVYGGASIGLMGQVAESVLAHGGEAVGVIPEFMLDYEVAHAGLTELHVVETMHERKAMMAERASAFIALPGGLGTFEEILEIATWGQLNQHQKPMILYNVNGFYNALIAQLDHAVQEGFLPLQHRAKLIVCEEIDQIYNVIKNLKMPKRFVV 5ITS Chain:D ((6-196)) ----------------DAPTLQRVTVFTGSALGSSSLYTQAAQTLAKTAVDRGIDLVYGGGKVGLMGIVADAFLESGGEAFGVITESLMKGELGHEKLTELEIVPDMHIRKRRMAELGDGFIAMPGGAGTLEELFEVWTWQQLGIHQKPVALYDVDGFWQPLLEMLEQMTQRGFIKRDFFECLIVESDPHALLKAMQTWTPPAPKWL

General information: TITO was launched using: RESULT: Template: 5ITS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 966 -145192 -150.30 -760.17 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain D : 0.78 3D Compatibility (PKB) : -150.30 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.581

(partial model without unconserved sides chains): PDB file : Tito_5ITS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ITS-query.scw PDB file : Tito_Scwrl_5ITS.pdb: