Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQHLSLPKDKIRFLLLEGVHQNAVDTLNAAGYTNIDYRKTALEGEALKEAIKDAHFIGIRSRTQLTEEVFEAANKLIAVGCFCIGTNQVDLKAAMARGIPVFNAPYSNTRSVAELVLAETILLLRRVPEKSAACHRGGWDKSAVGSFETRGKTLGIVGYGSIGSQLSVLAESMGMNVIYYDAVTKLPMGNARQVGSLDELLATADVVTLHIPDVPSTRNFFTKEQFAKMKEGAIFLNAARGTCVVIEDLADAIKSGHLAGAAVDVFPKEPKANGEEFQSPLRGLDNVILTPHVGGSTMEAQANIGLEVAEKFVAYSDKGMTLSAVNFPEIALPLTAGQHRLLHIHKNVPGVLSKINNLFAEQGINISGQSLMTKGDIGYLVMDVDA--SASQEALDMLHEVEGTIRVRVLF 2P9C Chain:B ((6-410)) ------LEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDDEQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGAFAIGTNQVDLDAAAKRGIPVFNAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYGHIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTKNMMGAKEISLMKPGSLLINASRGTVVDIPALADALASKHLAGAAIDVFPTEPATNSDPFTSPLAEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPLHVGR-RLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEKALQAMKAIPGTIRARLLY

General information: TITO was launched using: RESULT: Template: 2P9C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2137 -240061 -112.34 -595.68 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.91 3D Compatibility (PKB) : -112.34 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.669

(partial model without unconserved sides chains): PDB file : Tito_2P9C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P9C-query.scw PDB file : Tito_Scwrl_2P9C.pdb: