Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEFTGMSNQRIRIRLKSFDHRLIDQSAQEIVETAKRTGAQVCGPIPMPTRIERFNVLTSPHVNKDARDQYEIRTYKRLIDIVQPTDKTVDALMKLDLAAGVDVQIALG 5ME1 Chain:J ((4-102)) --------QRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPTRKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL-

General information: TITO was launched using: RESULT: Template: 5ME1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain J - contact count / total energy / energy per contact / energy per residue : 375 -39695 -105.85 -400.95 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain J : 0.91 3D Compatibility (PKB) : -105.85 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.717

(partial model without unconserved sides chains): PDB file : Tito_5ME1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ME1-query.scw PDB file : Tito_Scwrl_5ME1.pdb: