Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNERIYQVLKGLVFSEKAQVLGDTAGVQVFKVDINATKLEIKKAVEKLFGVEVVKVNTTITKGKTKRFGRTLGRRSDVKKAYVTLKAGQDVEMADLGDTAESAAE 5GAD Chain:U ((2-95)) IREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIKAAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDF------------

General information: TITO was launched using: RESULT: Template: 5GAD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain U - contact count / total energy / energy per contact / energy per residue : 347 -28367 -81.75 -301.78 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain U : 0.82 3D Compatibility (PKB) : -81.75 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.731

(partial model without unconserved sides chains): PDB file : Tito_5GAD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5GAD-query.scw PDB file : Tito_Scwrl_5GAD.pdb: