Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRHRNSGVKLGRTSSHRKAMFQNLANSLFEHELIKTTLPKAKELRRVAEPLITLAKNDTVANRRLAFARTRNAATVGKLFTVLGPRYKERNGGYLRVLKAGFRAGDAAPMAYVELVDREVNTSAE 5GAE Chain:O ((1-118)) MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKELRRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGFRAGDNAPMAYIELVDR-------

General information: TITO was launched using: RESULT: Template: 5GAE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain O - contact count / total energy / energy per contact / energy per residue : 406 -40003 -98.53 -339.00 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain O : 0.91 3D Compatibility (PKB) : -98.53 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.549

(partial model without unconserved sides chains): PDB file : Tito_5GAE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5GAE-query.scw PDB file : Tito_Scwrl_5GAE.pdb: