Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSSNTSRIAVQQIDPHELKAWIKAQALDLGFADCVSAKPDAQEQ-MPRFLEYLERGYHADMTYLEENLEKRADPTLLVPGTKSIICVRMNYLVESPKPRYVPFEPNSAITARYARGRDYHKVMRGRLKTLATRIREKVGDFESRPFADSAPIFEKSLAESAGMGWTGKHTLLIHKKSGSLFVLGELFTSLDLPFDEPATSHCGSCSACIDICPTQAIVEPYTLDARRCIAYLTIEYKGIIAEELRAGIGNRIFGCDDCQLICPWNSFAKTASIPDFNPRHGLDNISLLDIWQWDEATFLANTEGSPIRRTGYQSFKRNIAIGLGNAPYSKEIVDQLNKGKS-LHDEIVNVHIDWAIEQQLNQL 5D08 Chain:B ((30-373)) --------------NVYQLKEELIEYAKSIGVDKIGFTTADTFDSLKDRLILQESLGY--LSGFEEPDIEKRVTPKLLLPKAKSIVAIALAYPSRMKDAPRSTRTERRGIFCRASWGKDYHDVLREKLDLLEDFLKSKHEDIRTKSMVDTGELSDRAVAERAGIGFSAKNCMITTPEYGSYVYLAEMITNIPFEPDVPIEDMCGSCTKCLDACPTGALVNPGQLNAQRCISFLTQ-TKGFLPDEFRTKIGNRLYGCDTCQTVCPLNKGKDFHLHPEMEPDPEIAKPLLKPLLAISNREFKEKFGHVSGSWRGKKPIQRNAILALAHFKDA-SALPELTELMHKDPRPVIRGTAAWAIGKIGD--

General information: TITO was launched using: RESULT: Template: 5D08.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1800 -3511 -1.95 -10.27 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -1.95 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.445

(partial model without unconserved sides chains): PDB file : Tito_5D08.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5D08-query.scw PDB file : Tito_Scwrl_5D08.pdb: