Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITQNKDILFRSPIMNKLMVYAYTGVVITMLFWGSAFNAMSYVIQYMPPLSAAAERFTIASLGLFILFAAIGKLSWAALRQNLVIYLIIGIIGIAGFNLGCFYGLQTTSAVNGALIMATTPLITLLLTILLDGEKLTLNKFIGVVFGLSGVLFVISHGHISTLLHLKIAIGDLFILLGGVSFCLANVLSRRYVKNATPLETTTFSMMFGALTLIILSVIFEHPSQAIAMAPITAHLAMGYVIICSTMIAYLFWFNGIQKLGAGRASIFFNFVPVFSMLVALLAGQSLNIWQLIGTALVMLGVMSSGGFIQIKRPALIAKPCTK 5I20 Chain:A ((2-290)) ---------------SRSSATLIGFTAILLWSTLALATSST---GAVPPFLLTALTFTIGGAVGIAAGLARGV---SVLRQPWPVWVH-GIGGLFGYHFFYFSALKLAPPAEAGLVAYLWPLLIVLFSAFLPGERLRPAHVAGALMGLAGTVVLLGARAGGFGFAPEYVPGYLAAAACAVIWSVYSVASRRFAR------VPTEVVAGFCLATAALSALCHILFEPSVWPVGSEWLAVVALGIGPVGIAFYTWDIGMKRGDVRLLGVLSYAAPVLSTLLLVVAGFAAPSGALAIACALIVGGAAVATLLARRLES--------

General information: TITO was launched using: RESULT: Template: 5I20.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1413 -225583 -159.65 -786.00 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -159.65 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.284

(partial model without unconserved sides chains): PDB file : Tito_5I20.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I20-query.scw PDB file : Tito_Scwrl_5I20.pdb: