Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKPIVLVLSTLMLGMSATAMADSNHRWDNYHGKDHNRYEQRHDYRYDHRNDHRAPVWSHSERGNHYGRYVAFKGSDRIPVEYRNSRYYVSDWRAHRLYAPPRGYHWVKTPNQYLLVDSKHHIYRVR--------- 2L1S Chain:A ((1-83)) -----------------------------------------------------GAAGIDQYALKEFTADFTQFHIGDTVPAMYLTPEYNIKQWQQRNLPAPDAGSHWTYMGGNYVLITDTEGKILKVYDGEIFYHR

General information: TITO was launched using: RESULT: Template: 2L1S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 277 -29704 -107.23 -401.41 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -107.23 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.396

(partial model without unconserved sides chains): PDB file : Tito_2L1S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2L1S-query.scw PDB file : Tito_Scwrl_2L1S.pdb: