Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------MPIYNAPLAD---MKFILNDVFKAEQFWQANEKLAHVDAATAEAILEEMAKFAQNVTHPLNRTGDEEGARYENGEVFTPAGFKDAFRQYAEGGWIGLGADEEWGGQGMPKMLTVLADEMLFATNPSFMLYPLLSVGAGMALSS----YASQEQKETYLPKIYS----GEWSGTMCLTEPHAGTDLGI-IKTKAERNEDGTYNITGTKIFITGGDNDLAENIIHLVLAKTPD----APAGSRGISLFIVPKYLVNEDGSLGDRNHVGPGSIEHKMGIKASATCVMNFDGAKGYLVGKENEGLAAMFVMMNYERLSMGIQGLGASEFAYQNAAQYATARLQGRSAAGVQSPSKPADSILVHGDVRRMLLNVRANNEASRAFAVYVGQQLDITKFSTDAEAVKKANDRVALLTPIAKAYLTDTAFQATLDAQMVFGGHGYIREWGMEQCIRDLRIAQIYEGTNGVQSQDLIGRKTIKCGGAFIAEYITEIRDFANDLDTDLNFIKDATLDAATEIEAITQFIIEQAAENVEFPNSAAVDYLHAVGLLSFSYMFAKIAAAAKDKSGDFYQNKLALAQYFVERILPDIDARIAKIKAGSDLIMGFSEDYFTNQA 4Y9J Chain:A ((1-592)) TITARHTQYSHAKTGGFSQTGPTLHNPYKDDPILDRTLRRLLPESEYMRVAADLSKFGDRITSE-VEHLGRQAELEQPRLEHQ-DAWGKRVD--KLIVCNEWHKLKQICAEEGVISIGYEDS--VDPFVRRIHQVAKLFLFSPSAGLVSCPMAMTDGAVKTLTSLNLYGKHKLATEAVDRLRSRDPSKAWTSGQWMTEKKGGSDVAGGCDTYAVQIDKDTYRLHGYKWFSSAVDAD-----VALTLARIVDSDGNALEGSRGLSLFLLK--IRDESGNL---NGIQMVRLKNKLGTKQLPTAELLLDGAIAERIGDQGRGVAGISNMLNITRIHNAVASLGYMRRIISLARDYSTKRVVFG-----QTQSK-------WPLHTTTLAKMEVDTRGSMLLLFEAARLLGLSEAGKSSDVEAMM---LRLITPVLKLYAGKQAVPMVSEGIECFGGQGYMEDTGLPTLLRDAQVTPIWEGTTNVLSLDVL--RVFSGKENILLAFGKRVEQLLGNTKTEDEKLKKSKEAVESALKQLQKLLVKASDSAIQGETRIDSVARHIAFTIARIYSGALLIDHASDSSVANQSDIEVAYRYCCEQPLIDLRWEWFA----SERVKADREIVFDNFT

General information: TITO was launched using: RESULT: Template: 4Y9J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2953 44154 14.95 79.41 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 14.95 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.526

(partial model without unconserved sides chains): PDB file : Tito_4Y9J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Y9J-query.scw PDB file : Tito_Scwrl_4Y9J.pdb: