Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPQYKAPLRDMQFVLHELLNAEEHYAKLPDFQGNVSRELVDQYLEAAANFCENELSPLNQVGDREGCTWNDGVVTTPTGFKEAYQKYIELGFPSLSAEEQYGGQGLPNSLGIAISEMVGSSNWAWGMYPGLSH-GAVRTLEHHGSEEQKNTYLPNLVSGVWTGTMCLTESHAGSDLGIIRTKAEPNA-DGSYAISGEKIFISAGEHDMAENIIHIVLARLPGAPKGTKGISLFIVPKFNVNADGTLGERNAVRCGSIEHKMGIHGNATCVINFDQ---AKGYLIGPENRGLNCMFTFMNTARIGTAVQGLSASESSFQGALTYAKERLAMRSLSGPKAPEKEADPIIVHPAVRNMLLTQKAFAEGGRALVYLLAQYADIVEKGET-EEERKFADNILSLLTPIAKAFLTETGSESAKHGVQVFGGHGFISEHGMEQIVRDTRIACLYEGTTEIQALDLLGRKVLQTQGAMLRDFTKIVHKFVEANKDNAAMKEFVEPLAALNKEWGD----LTMQIGMRAMQ-------NPDEVGAAAVDYLYFSGYVTLAYLWARMALVAQEKLAAGTTDVDFYNAKVTTARFYFKKILPRVRSHVDVIAGGLDPLMALDAEHFAF 2Z1Q Chain:B ((8-526)) ------WQKGGGWLLEV---P-ERVYTPEDF----D-ESVKEIARTTRTFVEREVLPLLERMEHGELEL-------N---VPLMRKAGELGLLAIDVPEEYGGLDLPKVISTVVAEELSG-SGGFSVTYGAHTSIGTLPLVYFGTEEQKRKYLPKLASGEWIAAYCLTEPGSGSDALAAKTRATLSEDGKHYILNGVKQWISNAGF--AH--LFTVFAKVDG-----EHFTAFLVERDT----------PGLSFGPEEKKMGIKASSTRQVILEDVKVPVENVLGEIGKGHKIAFNVLNVGRYKLGAGAVGGAKRALELSAQYATQRVQFG------------RPIGRFGLIQQKLGEMASRIYAAESAVYRTVGLIDEALLGKKGPEAVMAGIEEYAVEASIIKVLGSEVLDYVVDEGVQIHGGYGYSQEYPIERAYRDARINRIFEGTNEINRLLIP-GMLLRRA-------------------------EPEDLELHQVQNLKKLALMVAGLAVQKYGQGVEEEQEVLGAVADILIDAYAAES----------ALLRARRLGGL----------APVLARIYLAQALDRAQAGAL-------------------

General information: TITO was launched using: RESULT: Template: 2Z1Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2662 -58693 -22.05 -122.79 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : -22.05 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.456

(partial model without unconserved sides chains): PDB file : Tito_2Z1Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z1Q-query.scw PDB file : Tito_Scwrl_2Z1Q.pdb: