Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKNRFFNTMQIQNQVQLKPFNTLSLDVTASHYTKVKSIEDIEEALAFAKEHELNVLVLSGGSNMLLPQQINALVIHLDIQGIDVLSEDQDFIRVKVGAGQVWHDFVLYTTKQNWFGLQNLALIPGLVGASPVQNIGAYGVEVGEFIESVQVYDRLLKQTGSISAADCHFSYRHSIFKDE-PARYIITHVTFKLLKQANLKLNYGDLKQAVGDNLTA-ENLQNQVIHIRQSKLPDPKEYPNVGSFFKNPIVNTQEFERLIAQFSTIPHYPQANGNVKIAAGWLIDQAGWKGKQLGVVGMFHKQALVLVNYANASLADVKKTYQAVQHDVEQRFQIMLEPEPVLYNNLGLIENHTE 2MBR Chain:A ((1-335)) ---------------HSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVLILGEGSNVLFLEDYRGTVIINRIKGIEIHD-EPDAWYLHVGAGENWHRLVKYTLQEGMPGLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDSVELATGKQVRLTAKECRFGYRDSIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNA-VCHMRTTKLPDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKGMQIGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGEVSA---

General information: TITO was launched using: RESULT: Template: 2MBR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1867 -104955 -56.22 -315.18 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -56.22 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.525

(partial model without unconserved sides chains): PDB file : Tito_2MBR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MBR-query.scw PDB file : Tito_Scwrl_2MBR.pdb: