Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEAQYKVLCVCLGNICRSPTAEVVFRHYCDQHQLNIIVDSARTSNYHPNKAPDQRSQLHAKKRGYDLSSLRARQLSTQDFLDFDLILAMDHQNFDDIHDLLQRAIFQFGSHQIRAKVALMSEHDPQYPQQALPDPYYGGDEGFERVLDQCESSALAWINILKKQLNV 4LRQ Chain:B ((1-150)) ---MQKVLVVCMGNICRSPTAEAVLRAKAAQLKVDVEVDSAGTIGYHQGNPPDARSKAAGEKRGYSFSGIKARKIRDEDFVKFDWILAADQENLAELKARCPQSH--------QHKLSLMLSHSDS-EYQEIPDPYYGGERGFELVLDLVEDAAEQFLLKLK-----

General information: TITO was launched using: RESULT: Template: 4LRQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 820 -76219 -92.95 -508.12 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -92.95 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_4LRQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LRQ-query.scw PDB file : Tito_Scwrl_4LRQ.pdb: