Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKSFFPSPRYFFISVVIWLALNMVLWYTGGDHWGQYLGFPQGYADAELPIGVSRFWSPAFLWFYLWFLVSTALFAGFWKIISNNPWQRWSIWGAAFILFNIWFAVQVNVAINAWYVPFWDLIQKMLSSGGGDISTLYSETLVFLYIAMVYVTLGVITA----FFTSHYVFRWRTAMNEYYTEH---WEKLRHIEGASQRVQEDTMRFATIMEDLGVELVKSVITLIAFLPILFHLSKYVLVLPLVGELNHSLVWAAIVWSIFGTVLLMVVGIKLPGLQFNNQKVEAAYRKELVYGEDHAE--RA--QPVTLKELFANVRKNYFRL----YFHYAYFNMTATWYGQLDILYNLVVLFPSIAAGKLTLGLMQQIMNVFGRVRDSFQYLISSWKTIIELLSIYKRLKAFESILHK 5U1D Chain:B ((156-450)) --------------------------------------------------------------------------------------------------FFFLVLAVLGETLIPHYSGRVIDILGGDF-----DPHAFASAIFFMCLFSFGSSLSAGCRGGCFTYTMSRINLRIREQLFSSLLRQDLGFFQETKTGELNSRLSSDTTLMSNWLPLNANVLLRSLVKVVGLYGFMLSISPRLT-------------LLSLLHMPFTIAAEKVYNTRHQEVLREIQDAVARAGQVVREAVGGLQTVRSFGAEEHEVCRYKEALEQCRQLYWRRDLERALYLLVRRVLHLGVQMLMLSCGLQQMQDGELTQGSLLSFMIYQESVGSYVQTLVYIYGDMLSNVGAAEKVFSYMDRQ--

General information: TITO was launched using: RESULT: Template: 5U1D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 726 -93063 -128.19 -332.37 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.56 3D Compatibility (PKB) : -128.19 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.355

(partial model without unconserved sides chains): PDB file : Tito_5U1D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5U1D-query.scw PDB file : Tito_Scwrl_5U1D.pdb: