Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSLSQVNADVLKQAQQRIQQDLLTTLAAFSIPEPLKSAVHHAVMLGGKRVRPALCYATASLQDNPNFAAARRAAVAVELIHCYSLAHDDLPCMDNDLLRRGQPTCHVAFGEDTALLAGDILQSMAFEVLGSRLFDEQGQGTDAAIVLKQIQILATASSKMVCGQVLDLQAEAKQISQDELENIHRNKTGALIQAAIMMGAVTIFPGTDQAIPKLRQYGQAIGLAFQVQDDILDITSSTETLGKTAGKDEQVQKSTYPALMGLEQAQIYAKELHDQAFEALAHFGENAKELVEISQFLLARTN 4F62 Chain:A ((13-292)) ---------------QSRVDQYLEQQLSDYAPANQLHNAMRYSLF-GGKRIRPMLTYASAQLVGDIS-SLTDASAAALESIHAYSLIHDDLPAMDN-------PTCHIQFDEATAILAGDALQTFAFELLS----NPTSAQPELAIKLIQELVVASGRNGMITGQMIDLSSENKNISLAELEQMHVHKTGALIKASVRMGALSTGQVKPEQLAKLDAYAHAIGLAFQVQDDIIDLT----------------NKATYPKLLGLDGAKALVVRLHEQAIAQISEFGDKSQPLTDLANYIID---

General information: TITO was launched using: RESULT: Template: 4F62.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1251 -135941 -108.67 -531.02 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -108.67 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.541

(partial model without unconserved sides chains): PDB file : Tito_4F62.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4F62-query.scw PDB file : Tito_Scwrl_4F62.pdb: