Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPDENNAMIRILIFISALLFF------IRFGNAEDIFNGTILFKNNDWHFVRCSIT--QDDYLIEAPQEIFTQFKELQQQQKNYWVSVLAEVNEQQNGNLILKIEKIDEVHLDETCHLLEALKNFENRE 4G12 Chain:A ((67-157)) -PNKESLLVELLVGVSARLLAGARDVTTRSANLAAALDGLIEF-HLDFALGEADLIRIQDRDLAHLPAVAERQVRKAQRQYVEVWVGVLRELN------------------------------------

General information: TITO was launched using: RESULT: Template: 4G12.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 208 -26735 -128.53 -322.11 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -128.53 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.315

(partial model without unconserved sides chains): PDB file : Tito_4G12.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G12-query.scw PDB file : Tito_Scwrl_4G12.pdb: