Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVQPNDYSKIYDIAVIGGGINGVGIANDAVGRGLSVFLCEKDDLASHTSSASSKLIHGGLRYLEYKEFRLVREALAEREVLLAKAPHIIKPMRFIMPHRPHLRPAWLIRAGLFFYDHLGKREKLLGSNRIYFKEDSPLKPAITRGFEYSDCTVDDARLVVLNALQAKEKGAKVVTRKRCVKAYRQQELWYLELQS--GAEFYQVRAKAIVNAAGPWVEEIISENLNLSSPYQIRLIQGSHIVVPKLYDCHKAFIMQNEDRRIVFAIPYLEKYTLIGTTDQEYTGDPQKVEITDVEIDYLLTVTNSHFKKQLTRADIVSQYSGVRALCDDESDNPSAITRDYTLALQAEDKTTPLLSVFGGKITTYRKLAEAALEHLAPFFNDMAEEWTADDPLPGA-ENWTTLEDLINQIKTRVSGISDSLANRWAHAYGTRVWNMLKERNAIEQLGQHFGHDLFECEVRYLCEYEWAHTAEDILWRRSKLGLA-FDEK-Q---VKVLEAYLSERRLKDDAA 2QCU Chain:B ((2-500)) ---------ETKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSASSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIRIGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVVRKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGMHLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYKGDPKAVKIEESEINYLLNVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDYTLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIEG-DRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAELKYLVDHEWVRRADDALWRRTKQG-MWLNADQQSRVSQWLVEYTQQRLSLA---

General information: TITO was launched using: RESULT: Template: 2QCU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2934 -44153 -15.05 -89.92 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -15.05 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.428

(partial model without unconserved sides chains): PDB file : Tito_2QCU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QCU-query.scw PDB file : Tito_Scwrl_2QCU.pdb: