Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIVADENLAFTDYFFSEFGDIQHKAGRTLTHTDVQDAEALLVRSVTAVNESLIQNTALKYVGSATIGTDHLDIQALEKQGITWANAAGCNAQAVAEYVITALLHLDASLLEQQEKFTLGIVGLGNVGKRLAYMAQLLGWKVIGFDPFVQLD--SIENVSFQTLLQQANAVSIHVPLTKKGEHATYHLFDEKAFAALQPNTILINSARGPVVKEAALIEDIQC-TQRKVVLDVFEHEPVISEGLLNMLALATPHIAGYSLEGKARGTQMIYEAFCQKFGYD-INKRFETQLPACED-Y--FSGHDLKAVLKQKLSQIYDIAQYDANIRACVKEGKVEQKAFDLLRKNYPLRREWAAHGGPQA 3OET Chain:E ((4-359)) MKILVDENMPYARELFSRLGEVKAVPGRPIPVEELNHADALMVRSVTKVNESLLSGTPINFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSALLMLAERDGFSLRDRTIGIVGVGNVGSRLQTRLEALGIRTLLCDPPRAARGDEGDFRTLDELVQEADVLTFHTPLYKDGPYKTLHLADETLIRRLKPGAILINACRGPVVDNAALLARLNAGQPLSVVLDVWEGEPDLNVALLEAVDIGTSHIAGYTLEGKARGTTQVFEAYSAFIGREQR-VALETLLPAPEFGRITLHGPLDQPTLKRLAHLVYDVRRDDAPLRKVAG----IPGEFDKLRKNYLERREWSSLYVMC-

General information: TITO was launched using: RESULT: Template: 3OET.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1835 -130041 -70.87 -372.61 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain E : 0.79 3D Compatibility (PKB) : -70.87 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_3OET.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OET-query.scw PDB file : Tito_Scwrl_3OET.pdb: