Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSILVIADHNNQTLNGATLNVVAAAQKIGGDITVLVAGSGAQAVADAAAKVAGVSKVLLADNAAYANQLAENVAGLVADLAKGYKYVLAASTTTGKNILPRVAALLDVSMITDIISVESANTFKRPIYAGNAIATVQSDEAIIVGTVRGTAFDPVAAEGGSAAVETVGEVKDAGVSKFVSEEIVKLDRPELTAARIVVSGGRGVGSGENYHKVLDPLADKLGAAQGASRAAVDAGFVPNDFQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKVIVAINKDEEAPINSVADYWLVGDLNAVVPELVSKL 1EFP Chain:A ((1-307)) -AVLLLGEVTNGALNRDATAKAVAAVKALGDVTVLCAGASAKAAAEEAAKIAGVAKVLVAEDALYGHRLAEPTAALIVGLAGDYSHIAAPATTDAKNVMPRVAALLDVMVLSDVSAILDADTFERPIYAGNAIQVVKSKDAKKVFTIRTASFD-AAGEGGTAPVTETAAAADPGLSSWVADEVAESDRPELTSARRVVSGGRGLGSKESF-AIIEELADKLGAAVGASRAAVDSGYAPNDWQVGQTGKVVAPELYVAVGISGAIQHLAGMKDSKVIVAINKDEEAPIFQIADYGLVGDLFSVVPELTGKL

General information: TITO was launched using: RESULT: Template: 1EFP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1825 -205760 -112.75 -670.23 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.88 3D Compatibility (PKB) : -112.75 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.489

(partial model without unconserved sides chains): PDB file : Tito_1EFP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EFP-query.scw PDB file : Tito_Scwrl_1EFP.pdb: