Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKIAVLVSGNGSNLQALIDA----NLSGQIVGVLSNKADAYALERAQNANIATAVISHKDFPSREDFDEAMHQQLIAWQADVVILAGFMRILTANFVDKWQGKMLNIHPSLLPAYKGVNTHQRVLNTGDRLHGCTVHFVTSELDAGQAIAQSAIEVKEHDNVASLAERVHKLEHFIYPQVAEWLCNGQLTW-KNGQ-AYFRNQILEHPIRFASW 1MEJ Chain:A ((14-211)) ---VAVLISGTGSNLQALIDSTREPNSSAQIDIVISNKAAVAGLDKAERAGIPTRVINHKLYKNRVEFDSAIDLVLEEFSIDIVCLAGFMRILSGPFVQKWNGKMLNIHPSLLPSFKGSNAHEQALETGVTVTGCTVHFVAEDVDAGQIILQEAVPVKRGDTVATLSERVKLAEHKIFPAALQLVASGTVQLGENGKICWV--------------

General information: TITO was launched using: RESULT: Template: 1MEJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 865 -39587 -45.76 -206.18 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -45.76 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.614

(partial model without unconserved sides chains): PDB file : Tito_1MEJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MEJ-query.scw PDB file : Tito_Scwrl_1MEJ.pdb: