Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEMMMKSIKHLAFPLLSAALVMTGCASRKPATTATTGTTNPSTVNTTGLSEDAALNAQNLAGASSKGVTEANKAALAKRIVHFDYDSSDLSTEDYQTLQAHAQFLMANANSKVALTGHTDERGTREYNMALGERRAKAVQNYLITSGVNPQQLEAVSYGKEAPVNPGHDESAWKENRRVEINYEAVPPLLK 4G4V Chain:A ((5-114)) --------------------------------------------------------------------------ALAKRVVHFDYDSSDLSTEDYQTLQAHAQFLMANANSKVALTGHTDERGTREYNMALGERRAKAVQNYLITSGVNPQQLEAVSYGKEAPVNPGHDESAWKENRRVEINYE-------

General information: TITO was launched using: RESULT: Template: 4G4V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 491 -13464 -27.42 -122.40 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -27.42 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.651

(partial model without unconserved sides chains): PDB file : Tito_4G4V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G4V-query.scw PDB file : Tito_Scwrl_4G4V.pdb: