Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIQRSGRFERIKKPLKSDMNVVPYIDVMLVLLVIFMVTAPMITSGIKVDLPQAN-NHPIESKDMP-AMVTIGKDGNIYLQYEDYKDNEPLSLEKL--ESVLSEAQKKAEQENKQLTVLVNGDKTRPYGEVVSLMASLQEAGLTQVGLLTEPLK 5BY4 Chain:A ((5-109)) ----------------------------------------PIITQSVEVDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVE--KDR----LERLPPEQVVAEVSSRF-KANPKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQP--

General information: TITO was launched using: RESULT: Template: 5BY4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 374 8645 23.11 85.59 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 23.11 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.710

(partial model without unconserved sides chains): PDB file : Tito_5BY4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5BY4-query.scw PDB file : Tito_Scwrl_5BY4.pdb: