Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQLSTIIEQAFEDCANFTAADCPSEIRQAVEEAIAGLDNGTLRVAEKINGEWVVHQWLKKAVLLSFKLNDNKPIESCDLRFYDKVETKFSGWTEEQFKAAGVRVVPPAVARRGSFQAKNVVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPLQANPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVYIGQSTRIYDRETGEIHYGRVPAGSVVVPGNLPSADGKYSLYAAIIVKKVDAQTRAKTSLNDLLRAD 3GOS Chain:A ((3-273)) MQQLQNVIETAFERRADITPANVDTVTREAITHVIDLLDTGALRVAEKIDGQWVTHQWLKKAVLLSFRINDNQVMEGAETRYYDKVPMKFAGYDEARFQREGFRVVPPATVRKGAFIARNTVLMPSYVNIGAFVDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPLQANPTIIEDNCFVGARSEVVEGVIVEEGSVISMGVFIGQSTRIYDRETGEVHYGRVPAGSVVVSGNLPSKDGSYSLYCAVIVKKVDA------GINELLR--

General information: TITO was launched using: RESULT: Template: 3GOS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1438 -210753 -146.56 -795.29 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.94 3D Compatibility (PKB) : -146.56 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.685

(partial model without unconserved sides chains): PDB file : Tito_3GOS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GOS-query.scw PDB file : Tito_Scwrl_3GOS.pdb: