Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKTSVIYPGTFDPITNGHVDLVTRASRMFDEVVVAIAIGHHKNPLFSLEERVALAQSSLGHLSNVEFVGFDGLLVNFFKEQKATAVLRGLRAVSDFEYEFQLANMNRQLDPHFEAVFLTPSEQYSFISSTLIREIARLKGDVTKFVPQAVVEAFERKHQQGW 5H16 Chain:A ((1-163)) MSKTRVIYPGTFDPITNGHVDLVTRASRMFDEVVVAIAIGHHKNPLFSLEERVALAQSSLGHLSNVEFVGFDGLLVNFFKEQKATAVLRGLRAVSDFEYEFQLANMNRQLDPHFEAVFLTPSEQYSFISSTLIREIARLKGDVTKFVPQAVVEAFERKHQQGW

General information: TITO was launched using: RESULT: Template: 5H16.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 745 -107058 -143.70 -656.79 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 1.00 3D Compatibility (PKB) : -143.70 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 1.00 QMean score : 0.666

(partial model without unconserved sides chains): PDB file : Tito_5H16.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5H16-query.scw PDB file : Tito_Scwrl_5H16.pdb: