Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence METLDSSIFDLTPIPMWIEDFSEVKQLFDLWRNQGVENLYEFLSQNENLVVECAHKIKIIKVNQKVLDLFEAKNQEELCANLNLIFKKEMFEAHIHELEALWNGKTHFSSTTINYTLSAQRIDVQLRGAILPGSETTFDRILITAEDITPYQNALRQEEKNRRLA-ESMFIYSPTSLWVEDFSRIKNRIDQLRLLGIEDFRTFLDVHPEFVR--QCIEDILILDVNQSTLDLFKAPDKTTLLKNTHKIFAEEMVETFREQLIELWQGNIHHKREAVNYALDGQIRNVLLQFTVFPGYEETWGLVQVALTDITARKKAENYLEYLGQHDVLTKLYNRAFFTEELNRLNRSIIRP----VSSIFLDMNGLKETNDQLGHDIGDGLLRRVGNILNQAIMNTAYTASRIGGDEFVILMPGADEANVLMMLQTIQELFNID--NQYYSSHPISIAIGHATTKENEQVEDMLKRADHHMYQKKKSYYQEILLR 4ZMU Chain:A ((5-345)) ----------MLACPLPPD---------EALRQQALDDM-----------------------------------------ALVDTPAEHYLDALVELARETFGVKTVLISLIDH--------------------DRQWFKARIG----------LDAEQTPRDLSFCGHAILASEPLMVTDASR-DPRFHDNPLVTGPPFIRFYAGEPLHASNGQAIGTLCLIDPSPRLLDLREGRQLNRLSILAEGYLQLR---------------SLTEHTRFLRQEIDREQRKSLLDP--------------------------------------LTQLWNRAGF-HALHQHELELARASDQRIGIIYSDIDHFKRINDTLGHRAGDSVLREAASRLRAA-LRPEDLLARFGGEEFVAMVRVRETTELTMIANRIRELMEATPIDCAGTSVPVTISAGCTLAGSGEEPERALARADAALYDAKRAGRNRVVSV

General information: TITO was launched using: RESULT: Template: 4ZMU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1753 -11934 -6.81 -35.94 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -6.81 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.316

(partial model without unconserved sides chains): PDB file : Tito_4ZMU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZMU-query.scw PDB file : Tito_Scwrl_4ZMU.pdb: