Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNMDTIEAKKNLNALCNEIEKLQNLSRSLMTAKEMLDIDAKIKRHKDQVKNIRSNLHA 5J0K Chain:B ((24-65)) -------LAKRLKELLRELERLQ---REGSSDEDVRELLREIKELVEEIEKL------

General information: TITO was launched using: RESULT: Template: 5J0K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 61 -11000 -180.33 -261.90 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : -180.33 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.708

(partial model without unconserved sides chains): PDB file : Tito_5J0K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5J0K-query.scw PDB file : Tito_Scwrl_5J0K.pdb: