Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAMLPEWTTACPDWEERIVAKKSLMPCEPLFPQVADVAERIFKELILVDVMGSPKMGDVTLDWVIEFVRAIFGSYNPNTKRRLIREFFLLISKKNTKSTIAAGVMLVALLLNDRLSAELIILAPTKEVADNSFNPIRDFIRADEELSAMINVSEHTKTVTHLGTG-------ATLKVIAAESNAAAGKKASIILIDEVWL------FGKRANAESMFREAKGGLASRPEGCVIYLSTMSDEVPCGVFKQLLDYARDVRDGIKEDKGFLPLIYEFPKRLVEAGEHLKPEHFYITNPNLGASVDLEYLISEFKKVKDAGEESLRDFLAKHLNIEIGMNLRANRWAGAEYWNKQKHVFGLDQL-IEQSDVITIGIDGGGLDDLLGYAVLGRLKKDPRVWWLWNHAWANKIALERRKENIPKYDDFKLEGSLTVVDRVGDDI--DQLAAIAKKVYDSGKLNKIGLDPLGLGGLLDGLLEAGIPEESMFAVPQGYKLMSYILTTERKLAEGNLYHAGQQLMTWAAGNARVVMVGNGMRITKQESGVGKIDPLIATFNAVALMSMNPEPTNKEYNVFFV--- 4BIJ Chain:A ((1-476)) MSTQSNRNALVVAQLKGDFVAFLFVLWKALNLPVPTKCQIDMAKVL----------------------------ANGDN------KKFILQAFRGIGKSFITCAFVVWSLWRDPQL--KILIVSASKERADANSIFIKNIIDLLPFLSELKPRPGQRDSVISFDVGPANPDHSPSVKSVGITGQLT-GSRADIIIADDVEIPSNSATMGAREKLWTLVQEFAALLKPLPSSRVIYLGTPQTE-------------MTLYKELEDNRGYTTIIW-------------PALYPRTREENLYYSQRLAMLRAEYDENPEALAGTPTDPVRFDRDDLRERELEYGKAGFTLQFMLNPNLSDAEKYPLRLRDAIVAALD---------------LEKAMHYQWLPNRQ------------NIIE--------DLPNVGLKGDDLHTYHDCSNNSGQYQQKIL---VIDPSGRGKDETGYAVLYTLNGYIYLMEAGGFRDGYSDKTLELLAKKAKQWGVQTVVYESNFGD--GMFGKVFSPILLKHHNCAMEEIRARGMKEMRICDTLEPVMQTHRLVIRDEV

General information: TITO was launched using: RESULT: Template: 4BIJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2304 -22584 -9.80 -49.63 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -9.80 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.204

(partial model without unconserved sides chains): PDB file : Tito_4BIJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BIJ-query.scw PDB file : Tito_Scwrl_4BIJ.pdb: