Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYKILAGVGSIVRTLFMVFTHGFRKRDTILYPEVPAEEIVPPRYRGRIILTRDPDGEERCVACNLCAVACPVGCISLQKAEKEDGRWYPEFFRINFSRCIFCGMCEEACPTTAIQLTPDFELGEYVRQDLVYEKENLLISGPGKYPDYNFYRVTGMAINGKEKGQAQKESAPIDVRSLLP 5LDW Chain:I ((30-158)) ----------LIRGLGMTLSYLFREPATINYPFEKGP--LSPRFRGEHALRRYPSGEERCIACKLCEAVCPAQAITIEAEPRADGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEFSTETHEELLYNKEKLLNNG---------------------------------------

General information: TITO was launched using: RESULT: Template: 5LDW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain I - contact count / total energy / energy per contact / energy per residue : 418 -65436 -156.55 -507.26 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain I : 0.71 3D Compatibility (PKB) : -156.55 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.326

(partial model without unconserved sides chains): PDB file : Tito_5LDW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LDW-query.scw PDB file : Tito_Scwrl_5LDW.pdb: