Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTLKNDRFLRALLREPVDTTPIWMMRQAGRYLPEYRETRSKAGDFLSLCKNTEFACEVTLQPLRRYDLDAAILFSDILTIPDALGLGLYFETGEGPKFHKTVRTEQDVANLPKLNAKADLDYVMNAVSTIRSALGGQVPLIGFSGSPWTLATYMVEGGSSKEFRFTKQMMYAQPEVLHALLDHLADSVIDYLNAQIDAGAQAIQIFDSWGGALAHREYVEFSLNYMNKIIAGLQREKDGRRIPVIVFTKGGGQWLEPMITTGADALGLDWTTPLNTARNVVSGRVALQGNLDPAVLYGSAASIEKAVKAMLDDAYANGEKTGYVANLGHGITQWVDPAQPKIFVDTVHEYSAKYLG 4ZR8 Chain:A ((11-365)) --TLKNDRFLRALLREPVDTTPIWMMRQAGRYLPEYRETRSKAG--LSLCKNTEFACEVTLQPLRRYDLDAAILFSDILTIPDALGLGLYFETGEGPKFHKTVRTEQDVANLPKLNAKADLDYVMNAVSTIRSALGGQVPLIGFSGSPWTLATYMVEGGSSKEFRFTKQMMYAQPEVLHALLDHLADSVIDYLNAQIDAGAQAIQIFDSWGGALAHREYVEFSLNYMKKIIAGLQREKDGRRIPVIVFTKGGGQWLEPMITTGADALGLDWTTPLNTARTTVAGRVALQGNLDPAVLYGSAASIEKAVKAMLDDAYANGEKTGYVANLGHGITQWVDPAQPKIFVDTVHEYSAKYLG

General information: TITO was launched using: RESULT: Template: 4ZR8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2065 -239974 -116.21 -679.81 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.99 3D Compatibility (PKB) : -116.21 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.99 QMean score : 0.574

(partial model without unconserved sides chains): PDB file : Tito_4ZR8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZR8-query.scw PDB file : Tito_Scwrl_4ZR8.pdb: