Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MINIQNTILGKIVDRKHEDLAARLKQRNLQDVEELAKAATPVRGFANALQHKRPGVIAEIKKASPSKGIIRADFNPAEIAQQYEQAGAACLSVLTDVDFFQGADENIAIARNHCTLPALRKDFLVDPYNVVEARALHADCILLIVACLSDQQLEEMSKTAFEHQLDVLVEVHDEEELERALKLSEQCLLGVNNRNLKTFDVDLNTTIRLKKLLPASRLLITESGIATPDDVRMMQEHDIHSFLVGESFMKQPRPDQAFTALFGQPQTV 1PII Chain:A ((1-255)) ----MQTVLAKIVADKAIWVEARKQQQPLASFQNEVQ--PSTRHFYDALQGARTAFILECKKASPSKGVIRDDFDPARIAAIYK-HYASAISVLTDEKYFQGSFNFLPIVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMGVLTEVSNEEEQERAIALG-AKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGINTYAQVRELSH-FANGFLIGSALMAHDDLHAAVRRVLLG----

General information: TITO was launched using: RESULT: Template: 1PII.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1432 -171940 -120.07 -674.27 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -120.07 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.435

(partial model without unconserved sides chains): PDB file : Tito_1PII.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PII-query.scw PDB file : Tito_Scwrl_1PII.pdb: