Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNKKNLGIGGIIVLLIAAYLGLDLSGHKNNPTPSSIPVNQRTETTFSNDGVDTIKAAYERRQSNVQVQGSGRVKAILREDNDGSRHQKFILVLKNGLSILVAHNIDLAP-KIEDLRKGDIVEFNGEYEYNEKGGVLHWTHHDPQNRHENGWLKHNGRIYQ 4QL5 Chain:A ((9-58)) --------------------------------------------------------------------EVEGKVVDTMPNA-------MFTVELENGHQILATVSGKIRKNYI-RILAGDRVTVEM-----------------------------------

General information: TITO was launched using: RESULT: Template: 4QL5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 158 -9629 -60.94 -196.50 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -60.94 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.724

(partial model without unconserved sides chains): PDB file : Tito_4QL5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QL5-query.scw PDB file : Tito_Scwrl_4QL5.pdb: