Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLSVGLKVANSLSLTPQLQQAIRLLQLSGLELEQEIQIQLDSNPLLEKVE--DELLAESLSTLEHKETDDLTTELNADHLPDDLPVDTEWDDIYTHQSTALGTPEFEEREDNRQVHLTLKEHILEQVNLLHFSKIDQLIAYCIVDALDDKGFLDAELEEIILAVQHLLSEMDIDEEVEEDEVLVVLKHIQRLDPIGIGARNLAECLKVQLEFLPRETEYLKEARSLLQ-YYELLIANDLNKLLKQTGLSKEQLKFAVDLLKTLKPYPGMDFEKQESEYQIPDVVVAKKDLHWQVQLNPDVMPKLRINSFYSSMIRRADQSDDNLYLRNQMLEAKNFIKSIDERHKTLLKVATCIVEHQKAFLEIGPEAMKPLVLRDVAEEVELHESTVSRVTTNKYMLTPRGLFELKYFFSSHVGTTTGGEASSTAIRAMIKKLVSNENPRKPLSDNAIAALLKEEGIEVARRTVAKYRESLHIPSSSERKVLI 5UI8 Chain:M ((20-474)) -------------------QQAIRLLQLSTLELQQELQQALESNPLLEQTDLHDE--------------------------------------------------------YQGETTQTLQDYLMWQVELTPFTDTDRAIATSIVDAVDDTGYLTIQIEDIVDSIG--------DDEIGLEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFAKETPWLEEARLIISDHLDLLANH--RTLMRVTRLKEEVLKEAVNLIQSLDPRPGQSIHTSEPEYVIPDVLVRKVSGRWTVELNADSIPRLKINQQYAAM-------ADGQFIRSNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELKYFFSS----------SSTAIRALVKKLIAAENPAKPLSDSKLTSMLSEQGIMVARRTVAKYRESLSIPPSNQRK---

General information: TITO was launched using: RESULT: Template: 5UI8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain M - contact count / total energy / energy per contact / energy per residue : 1301 43655 33.55 115.79 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain M : 0.79 3D Compatibility (PKB) : 33.55 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.538

(partial model without unconserved sides chains): PDB file : Tito_5UI8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UI8-query.scw PDB file : Tito_Scwrl_5UI8.pdb: