Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWKYLFSIFCLGANIHCYATDFGATNNFVSPNLQLKQNILPPTPKNIPLPAFGQRIIGWGTGAEGARQRLENIQPADVSMIKKQGTTLEMITAWQDFYEQEQQRNENNPTAKYRARLMKKIADLW 2MMB Chain:A ((2-107)) -------------------TDFGTTNNFVSPNLQLKQNVLPPTPKNIPLPAFGQRIIGWGTGAEGARQRLENIQPADVSMIKKQGTTLEMITAWQDFYEQEQQRNENNPTAKYRARLMKKIADLW

General information: TITO was launched using: RESULT: Template: 2MMB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 353 -32273 -91.42 -304.46 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.90 3D Compatibility (PKB) : -91.42 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.90 QMean score : 0.643

(partial model without unconserved sides chains): PDB file : Tito_2MMB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MMB-query.scw PDB file : Tito_Scwrl_2MMB.pdb: