Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLTELKKKPIGELIKIAEFMGLEGMARNRKQDIIFAILKRHAMNGEEIFGDGVLEILSDGFGFLRSAAGSYLAGPDDIYVSPSQIRRFNLRTGDTITGTIRPPKEGERYFALLKVNQINYDTPENSRNKILFENLTPLFPTEQLVMELGNGTTEDLTARVVDLVAPIGKGQRSIIVAPPKAGKTMLLQNIAQSIVRNNPEVFLIVLLIDERPEEVTEMERTVRGEVVASTFDEAPARHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGGVDAHALERPKRFFGAARNIEEGGSLTIISTALIETGSKMDDVIYEEFKGTGNQEITLDRRIAEKRVFPAMNIKKSGTRREERLMDEDKLRKVWILRKLLHPMDELAAMEFLLDRMKETKTNDDFFDQMKRKAST 1PVO Chain:D ((1-417)) MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAKSGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIRPPKEGERYFALLKVNEVNFDKPEN--NKILFENLTPLHANSRLRM----GSTEDLTARVLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTEMQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNTVVPA---VLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEEFKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGEIDAMEFLINKLAMTKTNDDFFEMMK-----

General information: TITO was launched using: RESULT: Template: 1PVO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2220 -202827 -91.36 -497.13 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain D : 0.94 3D Compatibility (PKB) : -91.36 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.516

(partial model without unconserved sides chains): PDB file : Tito_1PVO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PVO-query.scw PDB file : Tito_Scwrl_1PVO.pdb: