Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MARVKRGVVAHRRHKKILARTKGYYGARSRVYRVAFQAVIKAGQYAYRDRRQKKRQFRALWIARINAGARQNGLSYSRMIDGLKKAQVIIDRRVLADIAMHDAVAFAALAEKAKGALAA 5GAE Chain:R ((2-118)) -ARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQYAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFDKVAFTALVEKAKAALA-

General information: TITO was launched using: RESULT: Template: 5GAE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain R - contact count / total energy / energy per contact / energy per residue : 273 -41936 -153.61 -358.42 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain R : 0.95 3D Compatibility (PKB) : -153.61 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.95 QMean score : 0.629

(partial model without unconserved sides chains): PDB file : Tito_5GAE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5GAE-query.scw PDB file : Tito_Scwrl_5GAE.pdb: