Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFFRIFTIFLLILSTNIEAK-----EKLITGEKI---ASSY---KLLQSMTIPAIYENGMVLTKYANLYKMDNQCVIVSKLDNESNFGGYEDIIYFKNGIMLRSSKQSFFSIFLDDEAKIKSKEIKYGDFLNGKEVQKGLKEDFIQYRKKFNKYTLSKCS 3DIN Chain:C ((224-344)) ----LITIFGIILVQQAERRITIQYARRVTGRRVYGGASTYLPIKVNQGGVIPIIFASAIVSIPSAIASITNNET-----LKNLFRAGGFLYLLIYG---LLVFFFTYFYSVVIFDPREISENIRKYGGYIPG---------------------------

General information: TITO was launched using: RESULT: Template: 3DIN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 196 -10274 -52.42 -93.40 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain C : 0.66 3D Compatibility (PKB) : -52.42 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.022

(partial model without unconserved sides chains): PDB file : Tito_3DIN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DIN-query.scw PDB file : Tito_Scwrl_3DIN.pdb: