Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------MEEMSMRYV---SKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPFSTFSFENTTVLRETTYNLIVFFPFGVLLNV-----NFKRLSFSKKLGIIFLVSFLAE----------LIQFLFGIGVADITDLITNTTGGLIGLWA---YQLLNKHLSTNKLDRLAIILGYI-----------LFLFLIVAFLFLTRTVRLQ----- 5EIK Chain:A ((10-243)) IDHDTLIDAGGYVQKLKLYPYFDAAHYVLTCLSVRHDLGPDAISFSRKHPFSCWLSCMLMSFAGSFLSCFLLGEPIISPLKQ---HADILLGSIVWYLVFYSPFDVVFRLATWFPVKLGLSVLKEVQRTHKIAAGVKHAVRIYPESYLVQILVGVAKGAGSGVVKIVEQLARGTWHPTNHEILRPSFTTKACVIASIVFTLERHSMYVTAPHDLVYLCVVGFFIYFKLASLCLSVHD

General information: TITO was launched using: RESULT: Template: 5EIK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 586 -82702 -141.13 -489.36 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -141.13 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.168

(partial model without unconserved sides chains): PDB file : Tito_5EIK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EIK-query.scw PDB file : Tito_Scwrl_5EIK.pdb: