Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------------------------------------------------------MNIIVSWNYNDWRTIFMEDFIFELAIGLVFYFNQN-GKEAVNKKEE------------------------- 1DVO Chain:A ((33-184)) PPKWKVKKQKLAEKAAREAELTAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRDVLLEDVAQRNIPLSHKKLRRAMKAITR----------SESYLCAMKAGACRYDTEGYVTEHISQEEEVYAAERLDKIRRQNRIKAELQAVLD

General information: TITO was launched using: RESULT: Template: 1DVO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 5 820 164.00 23.43 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 164.00 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.153

(partial model without unconserved sides chains): PDB file : Tito_1DVO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1DVO-query.scw PDB file : Tito_Scwrl_1DVO.pdb: