Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGAS-VTYALSPILRKLYKDDDEYI---EALNNHFQYYN--TQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA 5TJA Chain:A ((1-212)) GSGLSNQLAVTFREENTIAFRHLF---LLGYSDGADDTFAAYTREQLYQAIFHAVDQYLALPDVSLGRYAYVRGGGDPWTNGSGLALCQRYYHRGHVDPANDTFDIDPMVVTDCIQV-----------------------DPPNLTLKFHKLVNVTIHFRLKTIN-----------------------------------LQSLINNEIPDCYTF-SVLITFD---NKAHSGRIPISLETQAHIQECKHPSVFQ---------------HG---

General information: TITO was launched using: RESULT: Template: 5TJA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 769 -39351 -51.17 -212.71 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -51.17 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.057

(partial model without unconserved sides chains): PDB file : Tito_5TJA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TJA-query.scw PDB file : Tito_Scwrl_5TJA.pdb: