Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKIAVEKVSAEEARLMSTGFLFPGQGSQKLGMLADIPEMY------MERLENVTGYRLV-----DKEENYQDTVFIQLALLTKAV-FYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVYHRAVLMKEAYPSGY-AMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSIEGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKNTDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKFGVDKGIRTINLDQHGIDDATFLYQKWR 2G1H Chain:A ((5-289)) --------------------FVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDLWALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCAGVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAE-GQVVSPVNFNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAKITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVGPGKVLTGL--TKRIVD------------------------

General information: TITO was launched using: RESULT: Template: 2G1H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1561 -3283 -2.10 -12.07 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -2.10 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.463

(partial model without unconserved sides chains): PDB file : Tito_2G1H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2G1H-query.scw PDB file : Tito_Scwrl_2G1H.pdb: