Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVGKMMFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEISTTQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRADAARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK 4EQF Chain:A ((75-189)) ------------------------------RLKEGDLPVTILFMEAAI-L--QDPGDAEAWQFLGITQAENENEQAAIVALQRCLELQPNNLKALMALAVSYTNTSHQ------QDACEALKN------------------

General information: TITO was launched using: RESULT: Template: 4EQF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 336 -12559 -37.38 -149.51 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -37.38 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.555

(partial model without unconserved sides chains): PDB file : Tito_4EQF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EQF-query.scw PDB file : Tito_Scwrl_4EQF.pdb: