Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------MKTELDLLKG--RSSDLPFKRFFCGISKLKNKKVFDFIY----ILKDEWQKLEKEKTALIYEEIEMIHKI-ENIRTIPNYIEAKIFFHH----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 4L9P Chain:B ((68-519)) VHPGIPALFREPPLIHDLLSTETTELQSETVNKCLPLLKGIHNSQKGPFNKY--GIPALQRKDHLEYLYDSLEDYPASFVALDASRPWMVYWALAGLCLLGEDVTRFRERVISTFTAAQNSTGGIGGGHGQMSHVASSYAAVLSIAMVGGEEAFKLIDRKAMWKWLGKLKQPDGGFTVCEGGEEDVRGAYCAMVVHALLDLPLALPPEAEARQNGLETFTDGLPEYLSRCQTYEGGISGSPGSEAHGAYAFCALACLCLLGRPEVVVPRYMNIATLLPWLSARQYAPEGGFSGRTNKLVDGCYSHWVGNCWPLVQAALDGTQPLAGPKRSSVGNLYSREGLTRYILSCCQCKLGGLRDKPGKHPDSYHTCYALTGLSTVQYYHYCTDSSVSSKDDFSSAFSWKHDPNFASDGQGSDIGVFTENDRLVPFHPIFVIPHKSAEDIRVWFENQSFDL

General information: TITO was launched using: RESULT: Template: 4L9P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 131 8999 68.69 112.49 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain B : 0.54 3D Compatibility (PKB) : 68.69 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_4L9P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4L9P-query.scw PDB file : Tito_Scwrl_4L9P.pdb: