Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MKNFLKIIPIPICGLILGLASLGNLFK---------DYHHVALGNLTGGISMILMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWLCAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFP--LIGKIFFWISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLVVALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYSSVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA 3M73 Chain:A ((1-313)) MNITKPFPLPTGYFGIPLGLAALSLAWFHLENLFPAARMVSDVLGIVASAVWILFILMYAYKLRYYFEEVRAEYHSPVRFSFIALIPITTMLVGDILYRWN--PLIAEVLIWIGTIGQLLFSTLRVSELWQGGVFEQKSTHPSFYLPAVAANFTSAS-LALLGYHD-GYLFFGAGMIAWIIFEPVLLQHLRI-SSLEPQFRATMGIVLAPAFVCVSAYLSINHGEVDTLAKILWGYGFLQLFFLLRLFPWIVEKGLNIGLWAFSAGLASMANSATAFYHGNV----------LQGVSIFAFVFSNVMIGLLVLMTIYKLTKGQFFL-------

General information: TITO was launched using: RESULT: Template: 3M73.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1480 -226880 -153.30 -758.79 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -153.30 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.340

(partial model without unconserved sides chains): PDB file : Tito_3M73.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3M73-query.scw PDB file : Tito_Scwrl_3M73.pdb: