Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVSTPCKLRGDDFMAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQEAQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILPAFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTLLPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKDIDTFNKELHV-------NKTLAVDEFGKVIIQSPKTRASRRVIS-----------LDTETLSILNNWKLQQKEEYLKLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPR-ITPHGFRHTHASLLLEAGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ 2A3V Chain:A ((2-320)) ---------GSQFLLSVREFMQTRY----------------------------YAKKTIEAYLHWITRYIHFHNKKHPSLMGDKEVEEFLTYLAVQGKVA--TKTQSLALNSLSFLYKEILKTPLS-------------------------------------------LEIRFQRSQLERKLPVVLTRDEIRRLLEIV----DPKHQLPIKLLYGSGLRLMECMRLRVQDIDFDYGAIRIWQGKGGKNRTVTLAKELYPHLKEQIALAKRYYDRDLHQKNYGGVWLPTALKEKYPNAPYEFRWHYLFPSFQLSLDPES--DVMRRHHMNETVLQKAVRRSAQEAGIEKTVTCHTLRHSFATHLLEVGADIRTVQEQLGHTD-VKTTQIYTHSGVLSPLSRL----------

General information: TITO was launched using: RESULT: Template: 2A3V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1150 -31626 -27.50 -107.57 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -27.50 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.388

(partial model without unconserved sides chains): PDB file : Tito_2A3V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2A3V-query.scw PDB file : Tito_Scwrl_2A3V.pdb: