Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYYFKQQILVSFINEKIILFS-CYVSLIN--------DVSLNKKADELNDQNLIIFVHQLLLKIFLLTINSWICKNIT 1O0W Chain:A ((12-73)) ---FQKETGINFKNEELLFRALCHSSYANEQNQAGRKDVESNEKLEFLGDAVLELFVCEILYKKY-------------

General information: TITO was launched using: RESULT: Template: 1O0W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 91 -8226 -90.40 -155.21 target 2D structure prediction score : 0.28 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -90.40 2D Compatibility (Sec. Struct. Predict.) : 0.28 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.290

(partial model without unconserved sides chains): PDB file : Tito_1O0W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1O0W-query.scw PDB file : Tito_Scwrl_1O0W.pdb: