Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEK-MKCPNCGKKFAYEEVNNVVEH---NDKEMPVVCPYCRTEAARIVTHGYFITEKIEDFLK 1MEY Chain:F ((1-38)) MEKPYKCPECGKSFS--QSSNLQKHQRTHTGEKPYKCPEC-----------------------

General information: TITO was launched using: RESULT: Template: 1MEY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 75 8175 109.00 240.44 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain F : 0.69 3D Compatibility (PKB) : 109.00 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.715

(partial model without unconserved sides chains): PDB file : Tito_1MEY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MEY-query.scw PDB file : Tito_Scwrl_1MEY.pdb: