Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNLEAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVPLAVAGLFLTMIVRTIAVPIVHLMDAA-AEEGNIRKVEMWHIIAVCLQGVRIAVPAAALLFIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAAISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY------- 5TPI Chain:A ((7-211)) QGLFSLGSLESTAAVR-IPSTLAHFNQRYPKIHLALSTGPSGTMIDGVL-EGALSAAFVD----GPLVHPGLEGLPVFPEEMMIVAPYG----HAPITRASEVNGANVYAFRANCSYRRHFESWFHADRATPGRIHEMESYHGMLACVI------AGAGLALIP----RSMLESMPGHQQ------------------VSAWPLAEEWRWLTTWLVWR--------------------------------RGAKTRQLEAFIALLNEDRQTVVSP

General information: TITO was launched using: RESULT: Template: 5TPI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1027 -153371 -149.34 -778.53 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -149.34 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.181

(partial model without unconserved sides chains): PDB file : Tito_5TPI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TPI-query.scw PDB file : Tito_Scwrl_5TPI.pdb: